badionotus, as well as on other species of water cucumbers, look in these rooked variety inside Colombia might have been limited by a few training on variety and you may shipments [dos, 16] and you may reproductive biology [17, 18]. not, you may still find of many openings in degree, eg regarding their reproductive designs and just how he could be influenced by details such as temperatures, salinity, water, and you may restaurants access.
The present study makes reference to areas of this new reproductive biology of your holothuroids I. badionotus and you may Isostichopus sp. regarding the Santa Marta Part with the gonadal directory (GI) and you may histological study of the new gonads. This research is meant to improve latest understanding of the fresh biology and you may ecology from sea cucumbers indigenous to the latest Colombian Caribbean Water and gives a scientific cause for the development of government strategies and preservation in the funding.
dos. Information and techniques
Specimens out of Isostichopus sp. have been gathered out of , when you find yourself specimens regarding Isostichopus badionotus were amassed out of on the Taganga Bay 
(11° 16′ ” N– 74° 11′ ” W) and you can Rodadero Bay (11° 12′ ” N– 74° 13′ ” W) regarding Caribbean region of Santa Marta, Colombia (Fig 1).
Additionally assesses this new influence off environment and you may chemical details (temperatures, water, brand new percentage of all-natural carbon dioxide stuff regarding the sediment) to their reproductive biology and you will analyzes the structure of the populations (volume from duration and you can weight, gender proportion, imply dimensions and you may pounds at first sexual maturity, fecundity)
Package into the inset Good is actually magnified in inset B; field in the inset B are magnified however committee. Source: Pointers feet INVEMAR.
Considering the wide array of shapes and colours that we. badionotus enjoys, this study concerned about the distinct organisms most abundant in common morphotype of this kinds contained in the research town, discussed by Vergara ainsi que al. when i. badionotus morphotype II.
Month-to-month 14 in order to 20 individuals of for each and every morphotype (I. badionotus and you can Isostichopus sp.) had been obtained of the snorkeling. For this purpose, a set of snorkelers swam a beneficial transect 5 meters large from the as much as 1000 meters a lot of time, twice. The original travel was created bordering brand new coastline line additionally the second far away of around ten m regarding shore range. The maximum research breadth was ten yards to have logistical and you will defense explanations and although chances are high you will find some one in the better point and depth, this may not assessed in today’s research. In the October, zero stuff would be made because of the passing of Hurricane Matthew, and that influenced the fresh new Colombian Caribbean in that day. All of the sea cucumbers was basically in person packaged for the plastic bags which have seawater and you will transported to your Aquaculture Lab of Universidad del Magdalena. The latest depth from bring of each private together with version of base they occupied were submitted (categorized given that rocky, sandy, coral, rock-red coral, rock-mud, rock-sand-coral). In addition, surface deposit products (which have a density maybe not greater than 10 mm) was indeed taken nearby the men and women to determine the fresh new part of all-natural carbon dioxide posts having fun with a size spectrometer, Euro EA 3000 elemental analyzer (EuroVector). Salinity and you may liquid temperature was indeed filed monthly, when you’re complete rainfall data have been taken from brand new details of your Simon Bolivar airport meteorological route of city of Santa Marta.
The individuals was indeed sacrificed about laboratory because of the position them inside seawater in the cuatro°C, which was in addition to intended to come to a maximum condition from contraction one to allowed to slow down the mistake throughout duration proportions, due to the capability that they have to expand or package from the tend to. Then adopting the human anatomy measurements was in fact pulled: full looks length (TL), using a flexible step one-meters recording scale on nearby 0.5 cm (shortly after lose by the thermal shock the new contracted body got a c or S shape, while the tape contoured your body, and so the total size is the profile length); gonad volume using a finished tube within nearby 0.1 cm step three ; body wall lbs (BW), identified as the extra weight of the individual instead viscera otherwise gonad and you can gonad lbs (GW) within nearby 0.1 grams. Gonad list (GI) is determined using the formula, GI = (GW/ BW)* 100 [19, 20].
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